Remove all invertebrates - these are extremely sensitive to copper and will not tolerate treatment with a copper-based medication. Turn off UV filters, ozone filters, and remove chemical filtration like MatrixCarbon and Purigen. Do not use in conjunction with any other medication. Do not use any products which contain reducing agents (conditioners, ammonia binders, etc.) while using Cupramine.
If the bottle has a dropper cap, use 20 drops (1 mL) per 40 L (10.5 US gallons) the first day, wait 48 hours, then repeat. On non-dropper caps, each inner ring is 1 mL. In freshwater use half dose. Final copper concentration is 0.5 mg/L (0.25 mg/L in freshwater). Leave at this concentration for 14 days. Do not redose without testing (MultiTest Copper). If tank has ever been treated with an ionic copper (e.g. copper chloride, sulfate or citrate), test copper level after initial dosing. Although most fish tolerate Cupramine to 0.8 mg/L, it is not advisable to exceed 0.6 mg/L copper.
Finish the Full Treatment
Ich, velvet, and many other parasites spend a large portion of their life cycle as a cyst that is immune to medication and hiding in the substrate of your tank. Remember that you need to finish the full treatment even if you can’t see parasites on the fish!
Cupramine can be removed using carbon or CupriSorb. Leave the copper-absorbing media in your tank for at least a week after the copper concentration has reached 0 to ensure all traces of the medication have been completely removed.